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Background: Plants are the rich sources of secondary metabolites such as alkaloids, phenols, flavonoids, tannins, saponins, glycosides, terpenoids etc. that possess a wide array of biological properties. Herbal formulation is the therapeutic medicine composed of medicinal plants having lots of phytochemical with pharmacological action. Quantitative analysis of these phyto-constituents is essential for the preparatory medicine. Trasina is a preparatory medicine extensively used to reduced stress and helpful to maintain body’s immunity. To create polyherbal compounds, the active ingredients of plants must first be extracted using a suitable solvent, the solution must then be evaporated, and the residue must then be adjusted to a specific standard. The Indian herbs Withania somnifera, Tinospora cordifolia, Eclipta alba, Ocimum sanctum, and Picrorrhiza kurroa, which are excellent under stressful circumstances, are the major ingredients in Trasina®, which is marketed as a multi-herbal capsule. Disease-curing phytochemicals included in natural therapies include flavonoids, glucosinolates, saponins, amino acids, monoterpenes, and others. Targets and goals: The purpose of this investigation was to find out if Trasina® included any phytochemical components. Methods: The material was extracted using ethanol, methanol, and aqueous solutions. Several phytochemical components were screened using a recognized technique. Results: In our study the multi herbal formulation (Trasina®) contained various phytochemicals like saponins, flavonoids, steroids, terpenoids, triterpenoids, alkaloids, sugars, anthraquinone, phenolic compounds, and glycosides. Conclusion: The phytochemical constituents of the material were found to include saponins, flavonoids, steroids, terpenoids, triterpenoids, alkaloids, carbohydrates, anthraquinone, phenolic compounds, and glycosides. The pharmacopoeial limitations were met for analytical research parameters such loss on drying, total ash, acid-soluble ash, and pH. The presence of active components may have therapeutic qualities significant for pharmacological activity, according to preliminary phytochemical screening
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