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The objective of the present study was to correlate the blood grouping with teeth gap. A total of 162 subjects participate about 38 were male and 123 were female and their mean age is between 18 and 22 years. I went to laboratory for the identification of my blood group and then through experiment, I came to know that my blood group is B+. I came to know that teeth gap is maximum in B+ and minimum in AB−, O−, and A−.